Fig 3. Absence of PKCα/β has no beneficial effects on RNA splicing defects caused by RNA toxicity.

(A) RT-PCR analysis of several RNA splicing targets associated with RNA toxicity, Clcn-1(exon 7a), Nfix1 (exon 7), Fxr1h (exon 15, 16), and Nrap (exon 12) shows that PKCα/β deficiency has no effect on splicing in the mice with RNA toxicity. (B) Quantification of the gels in (A) shows that RNA toxicity leads to splicing defects in the DM5 mice for all targets tested and PKCα/β deficiency has no effect on these splicing defects. For each groups, at least 4–5 mice were analyzed. *p = 0.05, Student’s t test; n.s. means not significant; error bars are mean±stdev.