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. 2016 Sep 22;11(9):e0163325. doi: 10.1371/journal.pone.0163325

Fig 6. Phospho-PKCθ levels are increased in the RNA toxicity mice.

Fig 6

(A, B) Western blot and quantitative RT-PCR shows no significant differences in PKCθ expression in uninduced and induced skeletal muscle tissues from RNA toxicity mice (n = 3–5 for uninduced and n = 3–5 for induced mice). Tubulin-loading control. (C) Western blot of skeletal muscle protein extracts shows increased levels of p-PKCθ in DM5 mice with RNA toxicity. n.s. indicates non-specific band; GAPDH-loading control. *p = 0.05, Student’s t test; error bars are mean±stdev.