Figure 4.

The regulation of p53 on the HOTAIR promoter.

Notes: (A) Two sequences shown at the top were two binding sites of p53 at the promoter of HOTAIR gene by bioinformatics prediction. Luciferase assay was carried out in A549 cells transfected with pGL3-basic or pGL-HOTAIR combined with pcDNA3-flag or flag-p53, respectively, for 48 hours. (B) The immunoprecipitation assay was performed to detect the activities of P53 binding to the promoter of lncRNA HOTAIR. The A549 cells were transfected with pcDNA-flag vector or flag-p53 expressing plasmids for 48 hours, and cell lysate was pulled down using IgG control or anti-p53 monoclonal antibody. Data represent mean ± SD of three independent experiments. **P<0.001.

Abbreviations: HOTAIR, HOX antisense intergenic RNA; IgG, immunoglobulin G; lncRNA, long noncoding RNA; SD, standard deviation; BS, binding sites.