Fig 3. Mutations in both the first and second KRAB motifs of ZFP568 affect ZFP568 ability to respond to TRIM28 levels.

Quantification of luciferase expression from a 5xUAS-luciferase reporter in HEK293T cells, in the presence of wild type and mutant versions of GAL4DBD-ZFP568 as indicated. (A) Luciferase expression in the presence of increasing amounts of transfected FLAG-TRIM28. (B) Luciferase expression upon TRIM28 depletion with siRNA. No siRNA and unspecific siRNAs were used as controls. Western blots show levels of TRIM28 as tested with α-TRIM28 antibodies. Luciferase expression is graphed as fold repression normalized to Gal4DBD (empty vector) and relative to wild type (column 1). Error bars represent standard deviation. Asterisks indicate samples which pairwise comparison had a p<0.005 (**). ns, no statistical significance.