Skip to main content
. 2016 Sep 22;14(9):e1002555. doi: 10.1371/journal.pbio.1002555

Fig 5. S50, S58, S62, S87, and S92 are modified by phosphorylation.

Fig 5

(A–E) Western blot (using an anti-EGFP antibody) of EGFP-D14 and its various single mutant (A, B), or EGFP-D14 and various double mutant protein derived from EGFP-D14_S50A (C), or EGFP-D14_S50,58,62A triple mutant protein (D), or EGFP-D14_S50,58,62,87,92A penta mutant protein (E) to analyse the gel mobility shift of these EGFP-D14 derivatives treated with (+) or without (-) CIP as shown. (F) Mass spectrum analysis of the EGFP-D14 peptide expressed by 293T cells. S50, S58, and S62 (upper panel), S87 and S92 (lower panel) five serine residues (pS) were identified to be modified by phosphorylation.