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. 2016 Sep 22;11(9):e0162947. doi: 10.1371/journal.pone.0162947

Fig 2. Assessment of qPCR Sensitivity for Bacteria Using S. epidermdis.

Fig 2

We assessed the sensitivity of quantitative PCR for detection of bacteria via amplification of the universal bacterial 16s ribosomal RNA gene, using DNA purified from germ free mice skin tissue spiked with serial 10-fold dilutions of quantified Staphylococcus epidermidis. The assay's sensitivity was at or better than 10 colony-forming units per mL, with no evidence of decreasing sensitivity at lower levels.