Figure 6.
Effect of P1 on 5-LOX product leukotriene B4 (LTB4) in RPE cells. (A) Quantitation of LTB4 levels in ARPE-19 cells treated with indicated concentrations of H2O2 and P1. (B) Quantitation of LTB4 levels in primary pig RPE cells treated with indicated concentration of H2O2 and P1. The levels of LTB4 were normalized to the total number of cells per well. Each condition was tested in duplicate wells per experiment ± SD (error bars). LTB4 level in each well was measured in triplicates. Each experiment was performed twice. *P ≤ 0.05.