Levels of anti-mitochondrial antibodies in ARE-Del−/− mice. 2A–B. Level of anti-PDC-E2, anti-OGDC-E2 and anti-BCOADC-E2 in the serum of female (A) and male (B) ARE-Del−/− at age 10 (±2) weeks (n=4). 2C–D. Level of anti-PDC-E2, anti-OGDC-E2 and anti-BCOADC-E2 in the serum of female (C) and male (D) ARE-Del−/− at age 20 (±2) weeks (n=7–8). Data represent mean ± SEM. Statistical analysis was performed by the unpaired Student’s t-test. * P < 0.05, ** P < 0.01, *** P < 0.001, n.s., not significant. All data are representative of at least three independent experiments.