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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Hepatology. 2016 Jul 22;64(4):1057–1071. doi: 10.1002/hep.28680

Figure 3. Functional inhibition of SYK suppressed alcohol-induced hepatic SYK activation and hepatic neutrophil infiltration.

Figure 3

C57BL/6 mice were fed a chronic alcohol diet for 10 days with a single ethanol gavage at the end (NIAAA model) or an isocaloric pair fed (PF) diet with a single sugar gavage and either SYK inhibitor (SYK Inh.) or vehicle treatment (+ Veh.). Total liver protein was analyzed for activated pSYKY525/526 by western blot (A). NF-κB nuclear binding was assessed from liver nuclear extracts by electrophoretic mobility shift assay (B). Total RNA was extracted from mouse livers and assessed by RT-qPCR for E-Selectin (C), Ly6G (C), and MPO (D). Liver samples were assessed by immunohistology for neutrophil infiltration by MPO staining (D). A p-value <0.05 was considered statistically significant by ANOVA for 6–8 mice per experimental group (*, p<0.05 compared to baseline; #, p<0.05 for EtOH + vehicle group).