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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Hepatology. 2016 Jul 30;64(4):1202–1216. doi: 10.1002/hep.28704

Figure 1.

Figure 1

Mast cells were found in close proximity to bile ducts in Mdr2−/− mice. (A) The number of mMCPT-1-positive mast cells (found close to large bile ducts) increased in Mdr2−/− mice compared to WT and treatment with cromolyn sodium decreased the number of infiltrating mast cells. (A) Mast cells are indicated with red arrows and large bile ducts are marked with black arrows. Images are 20× magnification. Histamine secretion was measured by EIA in serum from WT, Mdr2−/− and Mdr2−/− + cromolyn sodium. (B) Histamine levels increased in Mdr2−/− mice, but decreased in Mdr2−/− mice treated with cromolyn sodium compared to WT. (C) c-Kit, chymase and tryptase gene expression are increased in Mdr2−/− mice compared to WT, whereas treatment with cromolyn sodium decreased the gene expression in Mdr2−/− mice. Data are expressed as mean ± SEM of at least 6 experiments for real-time PCR and 12 experiments for EIA. *p<0.05 versus WT mice; #p<0.05 versus Mdr2−/− mice.