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Chemical structures and cell viability assay to determine IC50 values. A and B: Chemical structures of MMAE and MMAEp, respectively. C and D: Cell viability assay used to calculate the IC50 values of both compounds. Data are presented as mean ± SEM of three independently performed experiments. * p<0.01, ** p<0.001 compared to the control (0 nM). E: Calculated IC50 values for both compounds in PC-3 and C4-2B cell lines using linear regression analysis.
