Figure 6. Increased germ cell apoptosis in backcrossed KO testes during the first wave of spermatogenesis.
(A–F) Immunostaining was done to detect c-PARP1 (green), and phalloidin labeled F-actin (red). The c-PARP1+ cells were counted at each age and in each genotype, and the results are presented for each age below their respective panels as c-PARP1+ cells per mm2 of seminiferous cord (P8, P12) or tubule (P20). Asterisk indicates statistical significance (P<0.05). Scale bar = 30 µm.