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Breeding design for placing null alleles on 30 different genetic backgrounds. Males heterozygous for the null allele mutation (red asterisk) on a C57BL/6J background were bred with females from 30 inbred laboratory mouse strains (represented by arbitrarily selected colors). This generated 30 distinct F1s that were genetically identical except at the targeted allele, where they were either +/- or +/+.
