Figure 3. Hyperinsulinemic-euglycemic clamp.

Fasted (6 hr) HFD-fed mice received constant infusion of insulin and ³H-glucose tracer but variable glucose infusion rates to maintain blood glucose levels. Glucose infusion rate was recorded for 2 hr during the clamp, and blood was obtained before and after the clamp. Mice fed HFD and subsequent UCn2 gene transfer: (A) required higher glucose infusion rates (GIR) to maintain euglycemia during the clamp; (B) showed increased glucose disposal rate (GDR); (C) showed increased insulin-stimulated glucose disposal rate (IS-GDR), indicating increased insulin sensitivity; and (D) showed reductions in hepatic glucose production (HGP) before and after insulin stimulation. The relative reductions in HGP during insulin stimulation showed no group difference. HFD, high fat diet; UCn2, UC: urocortin-2 gene transfer.