Table 1.
The primers, strains and plasmids used in this study
| Primers, strains and plasmids | Sequence (5′–3′) or description | Source or reference |
|---|---|---|
| Primers for qPCR (Supplied in Additional file 1: Table S1) | ||
| Primers for gene knockout (Extended sequences for overlap are marked with underline) | ||
| EmrB-up-F | TTAACTAGACAGATCTATTTCTATTTTGGCTTGTCGTT | This study |
| EmrB-up-R | AAAATCCCTTAACGTGAGTTCCTTTTGTTGGCGTTTGC | This study |
| EmrB-down-F | CCGAAAAGTGCCACCTGAAATATGGTGGTCAAGAAGGCG | This study |
| EmrB-down-R | GGGCGATATCGGATCCCTGGATACAGCATGTGGAAAC | This study |
| EmrA-up-F | TCGATGCATGCCATGGAAATGTCAGCAACTTCTTCAGG | This study |
| EmrA-up-R | AAAATCCCTTAACGTGAGTTAATAAAAGCCAGCAATCCCAAT | This study |
| EmrA-down-F | CCGAAAAGTGCCACCTGAAATCCTGGAATGAACGCTGAAG | This study |
| EmrA-down-R | GGGCGATATCGGATCCAAATAGATACGCCGTAATTGGTA | This study |
| npt II-F | AACTCACGTTAAGGGATTTTGG | This study |
| npt II-R | TTTCAGGTGGCACTTTTCG | This study |
| Strains used in this study | ||
| S. aureus LZ-01 | S. aureus LZ-01 is the isolated objective strain | Zhang et al. (2013) |
| S. aureus ATCC 25923 | S. aureus ATCC 25923 is a purchased standard strain | Purchased from CCTCC |
| S. aureus RN4220 | Initial recipient for modification of reconstructed plasmids | Augustin and Götz (1990) |
| E.coli. Top 10 | E.coli. Top10 is used as a host for vector replication | University of Chicago |
| ΔemrB | ΔEmrB is S. aureus LZ-01 mutant strain with deletion of emrB | This study |
| ΔemrA | ΔEmrA is S. aureus LZ-01 mutant strain with deletion of emrA | This study |
| CB | S. aureus CB is the complemented strain for ΔemrB | This study |
| CA | S. aureus CA is the complemented strain for ΔemrA | This study |
| Plasmids | ||
| pMAD | pMAD is a shuttle vector used for gene knockout of S. aureus | Arnaud et al. (2004) |
| pET-30a | pET-30a used for clone of npt II gene | Purchased |