Table 3.
Genes involved in co-resistance of Cr(VI) and ampicillin were tested by RNA-seq and qPCR methods in strain LZ-01
| Genes | Gene annotation | RNA-seq (0.4 mM Cr(VI)) Fold change |
qPCR (0.2 mg ml−1 Amp) Fold change |
|---|---|---|---|
| Multidrug transporter and resistance associated genes | |||
| SAV0198 | ABC-type antimicrobial peptide transport system | 6.21 | 0.61 |
| SAV0199 | ABC-type antimicrobial peptide transport system | 10.14 | 0.72 |
| SAV0203 | ABC-type multidrug transport system | 7.92 | 0.86 |
| SAV0274 | Drug transporter | 5.78 | 0.57 |
| SAV0275 | Penicillin amidase V | 2.96 | 0.72 |
| SAV0276 | Peptidoglycan hydrolase | 1.58 | 0.47 |
| SAV0277 | ABC-type multidrug transport system | 1.14 | 0.79 |
| SAV0351 | ABC-type multidrug transport system | 1.07 | 1.18 |
| SAV0661 | ABC-type antimicrobial peptide transport system | 1.86 | 1.19 |
| SAV1035 | ABC-type antimicrobial peptide transport system | 8.23 | 0.30 |
| SAV1318 | ABC-type multidrug transport system | 5.10 | 0.97 |
| SAV1837 | ABC-type multidrug transport system | 1.01 | 0.99 |
| SAV1866 | ABC-type multidrug transport system | 1.23 | 0.71 |
| SAV2166 | Drug resistance MFS transporter | 1.47 | 0.58 |
| SAV2168 | Putative multidrug transporter | 4.26 | 1.05 |
| SAV2169 | Multidrug ABC transporter | 3.58 | 1.01 |
| SAV2261 | Cation/multidrug efflux pump | 1.25 | 1.13 |
| SAV2262 | Uncharacterized protein involved in methicillin resistance | 1.01 | 1.12 |
| SAV2265 | Mar R family transcriptional regulator | 10.64 | 0.67 |
| SAV2266 | MFS family transporter ybf D | 6.44 | 0.63 |
| SAV2352(emrB) | Drug resistance MFS transporter, drug:H+ antiporter-2 family | 2.14 | 2.34a |
| SAV2353(emrA) | Multidrug resistance efflux pump | 1.29 | 1.60a |
| SAV2355 | Drug resistance transporter, Bcr/Cfl A subfamily | 3.60 | 0.86 |
| SAV2419 | Toxin production and resistance | 1.28 | 0.94 |
| SAV2420 | Toxin production and resistance | 1.22 | 0.94 |
| SAV2421 | Toxin production and resistance | 1.51 | 0.59 |
| SAV2428 | ABC-type multidrug transport system | 15.80 | 0.51 |
| SAV2462 | Antibiotic resistance protein | 2.13 | 0.79 |
| SAV2552 | Predicted drug exports of the RND family | 4.55 | 0.66 |
| SAV2623 | ABC-type antimicrobial peptide transport system | 1.68 | 0.96 |
| SAV2701 | ABC-type antimicrobial peptide transport system | 13.05 | 0.73 |
| SAV2702 | Similar to ABC transporter, vraE protein | 10.66 | 0.63 |
| Ampicillin-resistant genes | |||
| SAV1275 | Metallo-beta-lactamase superfamily | 0.98 | 0.80 |
| SAV1504 | Metallo-beta-lactamase | 1.02 | 0.51 |
| SAV1545 | Metallo-beta-lactamase superfamily protein | 0.74 | 1.02 |
| SAV2441 | Similar to beta-lactamase | 1.42 | 0.44 |
| pbpA | Penicillin-binding protein 1 | 1.54 | 3.88 |
| pbp2 | Penicillin-binding protein 1A | 2.10 | 2.02 |
| pbp3 | Penicillin-binding protein 3 | 1.05 | 2.71 |
| pbp4 | d-alanyl-d-alanine carboxypeptidase (penicillin-binding protein) | 0.77 | 3.32 |
aStands for the selected genes for next study