Figure 5. LYVE-1⁺ macrophages are intimately associated with but do not incorporate into regenerated lymphatics.

Representative dual immunofluorescence localization of LYVE-1 and CD11b in the distal hind limb skin 4 weeks after DT administration. Regenerating lymphatic vessels are outlined in dotted yellow lines. LYVE-1⁺CD11b⁺ macrophages (yellow arrows) are near the outer surface of lymphatic vessels, while LYVE-1^–CD11b⁺ macrophages (white arrows) are intimately associated with them (scale bar: 100 μm).