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. 2016 May 19;1(7):e85911. doi: 10.1172/jci.insight.85911

Figure 5. Thymus-derived Tregs are recruited into the graft and prevent rejection of non-MHC antigens.

Figure 5

(A) Peripheral blood of secondary graft recipients (syngeneic n = 3; 0-Gy Tregs, n = 7; 3 Gy, n = 5; 9 Gy, n = 5) was analyzed for graft-derived CD4, CD8, and CD19 populations and (B) FoxP3+ cells among CD4+ populations (naive B6 were used as control). (C) Proliferation was measured by Ki67 expression in Treg (FoxP3+) and non-Treg (FoxP3–) CD4 populations. (D) Thymic origin of graft-derived Tregs was determined by Helios and Neuropilin-1 (Nrp1) coexpression in CD4+FoxP3+ cells (mean percentages + SD of indicated cell populations are shown; 2-tailed t test).