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. 2016 Jun 16;1(9):e87607. doi: 10.1172/jci.insight.87607

Figure 6. PRL-3 oncoprotein may be secreted out of cancer cells and act as bait for PRL3-zumab.

Figure 6

(A) Analysis of PRL-3 protein expression in intracellular protein pools (cell lysate) and extracellular protein pools (concentrated conditioned media) after culturing gastric cancer (GC) cells in serum-free media for 48 hours. For extracellular protein analysis, conditioned media (50 ml) from 5 dishes of GC cells were first cleared of dead cells and cellular debris, followed by centrifugal concentration (final volume ~0.2 ml). Mr, relative molecular mass (kDa). (B) Orthotopic SNU-484 and MKN45 tumor tissue cryosections from mice subject to various treatments were analyzed by immunohistochemistry for PRL3-zumab using anti-human IgG antibodies. Scale bar: 20 μm.