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. 2016 Sep 8;1(14):e88728. doi: 10.1172/jci.insight.88728

Figure 4. Surface-exposed mutations affect pig airway epithelial transduction.

Figure 4

(A) Diagram highlighting capsid mutations found in AAV2 or AAV2H22. Site-directed mutagenesis was used to introduce mutations to the AAV2 capsid (top) or to revert AAV2H22 to WT (AAV2) capsid sequence (bottom). (B and C) Effect of surface-exposed mutations on GFP transduction efficiency. Residues L598 and I708 were sufficient to augment AAV2 transduction efficiency (B) but were not required for AAV2H22 transduction (C). (D and E) Effect of non–surface-exposed mutations on GFP transduction efficiency. Residues A67 and V648, but not G207, in AAV2 were sufficient to augment GFP transduction (D). However, none were required for AAV2H22 transduction (E). n = 12; *P < 0.01 vs. AAV2 (B and D) or AAV2H22 (C and E). *P < 0.01, Mann-Whitney nonparametric t test.