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Binding of control 12-mer peptides (linker only, L33, and P33) to HLA-DRB3*01:01-positive APCs (D4BL4), measured by flow cytometry after peptide pulsing with AdEtOH as MLE. No increase was seen with linker only, while an increase can be seen with L33, LolP1, and P33. Experiments were conducted twice with two replicates; representative histograms are shown. AdEtOH, adamantane ethanol; APC, antigen-presenting cell; MLE, MHC-loading enhancer.
