Figure 4. Biofilm formation in skin tissue models infected with group A streptococal (GAS) necrotizing soft tissue infection isolates associated with biofilm or nonbiofilm.

Representative 3D reconstructions of immunofluorescence images of biofilm after 48 hours of skin model infections with strains from patients with biofilm-positive biopsies (2006 and 2028) or biofilm-negative biopsies (5004 and 5006) are shown (original magnification, ×100). GAS-specific antibody, wheat germ agglutinin (WGA), and Nile red were used.