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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Glia. 2016 Jun 3;64(8):1363–1380. doi: 10.1002/glia.23009

Fig. 1. LRP1 is LewisX glycosylated in the developing spinal cord.

Fig. 1

A. An overview showing frontal spinal cord sections of E12.5 C57Bl6 mouse embryos immunostained against the glycan LewisX epitope mAb5750LeX and mAb487LeX (red) co-localizing with LRP1 (green) and Nestin (blue) in the radial glia cells of the central canal, scale bar 100 μm. B. High magnification images of the central canal region, immunostained for the same markers as in A, confirming co-localization, scale bar 50 μm. C. Western blot detecting LRP1α-chain, 5750LeX and 487LeX epitopes in the E14.5 spinal cord protein lysate, immunoprecipitated with mAb B411E2, illustrating the LeX glycosylation of mature LRP1 in the developing spinal cord. IP = immunoprecipitation, AB = antibody.