A. Schematic representation of full-length receptor LRP1, wild type and mutated mini-receptors. I-IV = the ligand binding domains. B. A representative fluorescent image of neurospheres electroporated with GFP, illustrating high transfection efficiency. The result of the differentiation assays shows that LRP1 knockout NSPCs transfected with wild type mini LRP1 generated significantly more O4-positive oligodendrocytes compared to NSPCs transfected with mini-LRP1 including the mutated intracellular domain, or GFP-transfected cells (N = 4). Data are expressed as mean ± SE, (* indicates P < 0.05, ** — P < 0.01).