FIGURE 3.

T16Ainh-AO1 blocks the agonist-evoked calcium-dependent chloride current in pancreatic acinar cells but has no effect on zymogen granule acidification. A and B, whole-cell patch clamp with voltage clamp steps showing activation of a large current after stimulation with 10 pm CCK that is blocked when cells were stimulated in the presence of 10 μm T16AinhAO1, as shown in the current-voltage relationships (B). C and D, cells incubated in Lysosensor were two-photon-imaged with excitation at 750 nm and emission collected at wavelength >520 nm and <490 nm. Images were ratioed (C and D) and calibrated across a range of pH values (D). E, the cellular pH was not different (Student's t test) after treatment with 10 μm T16Ainh-AO1. Scale bar = 5 μm. pF, picofarad; ns, not significant.