FIGURE 6.

TMEM16A transports HCO₃⁻ into the lumen during supramaximal agonist stimulation. Addition of 10 μm T16Ainh-AO1 to HCO₃⁻-buffered solution significantly increased luminal acidification (B–D, see also supplemental Movies 15 and 16) compared with the HCO₃⁻-buffered control responses (A, C, and D, DMSO; see also supplemental Movies 13 and 14). Counts of the numbers of exocytic events per luminal length show that this is not altered in the presence of T16Ainh-AO1 (E). Scale bars = 5 μm. ns, not significant. Student's t test; *, p < 0.05.