FIGURE 7.

Niflumic acid also enhances luminal acidification in response to supramaximal agonist stimulation. Addition of 100 μm niflumic acid (NFA) to HCO₃⁻-buffered solution significantly increased luminal acidification (B–D, see also supplemental Movies 19 and 20) compared with the HCO₃⁻-buffered control responses (A, C, and D, DMSO; see also supplemental Movies 17 and 18). Counts of the numbers of exocytic events per luminal length show that this was not altered in the presence of niflumic acid (E). Scale bars = 5 μm. ns, not significant. Student's t test; *, p < 0.05.