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. 2016 Aug 8;291(39):20387–20401. doi: 10.1074/jbc.M116.743138

FIGURE 3.

FIGURE 3.

Concentration-dependent effects of 12-h treatment with PK on hERG channels expressed in HEK cells cultured in standard medium (MEM plus 10% FBS). A, concentration-dependent effects of PK for 12 h on IhERG. Left, hERG current traces in control (Ctrl) or 20 μg/ml PK-treated cells. Right, IhERG plotted against PK concentrations and fit to the Hill equation. EC50 = 5.9 μg/ml, Hill coefficient = 1.0. (Numbers above each dot represent number of cells examined from four independent treatments.) B, concentration-dependent effects of PK for 12 h on hERG expression. Left, representative Western blot demonstrating hERG expression after 12-h PK treatment at various concentrations. Actin is used as a loading control. M, molecular weight marker. Right, the 155 kDa band intensities normalized to the control (0) and fit to the Hill equation. EC50 = 8.4 μg/ml, Hill coefficient = 1.1 (n = 3). Error bars, S.E.