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. 2016 Aug 8;291(39):20387–20401. doi: 10.1074/jbc.M116.743138

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — Cardiac ischemia in rabbits reduces mature ERG protein expression and increases the expression of calpain, matriptase-2, and MMP-2. A and B, Western blots of ventricular tissue from control (Ctrl) or ischemic (Isc) rabbit hearts showing that 24-h ischemia significantly decreases the 155-kDa ERG expression (n = 3) but not KCNQ1 expression (n = 3). C–E, Western blots demonstrating that ventricular tissue from ischemic rabbit hearts contains significantly elevated levels of calpain (CAPN; n = 4), matriptase-2 (Matr-2; n = 4), and MMP-2 (n = 6) compared with control. Actin was used as a loading control. The band intensities are normalized to their respective controls and shown as relative values below corresponding Western blot images. *, p < 0.05 versus control; **, p < 0.01 versus control. Error bars, S.E.