FIGURE 8.

Low levels of δ subunit cDNA were sufficient to eliminate kinetic signatures of α1β2 receptors. GABA (1 mm; 4 s) was applied to HEK293T cells transfected with 1 μg of α1, 1 μg of β2, and varying amounts of δ subunit cDNA (A). Whole cell currents were recorded and analyzed to determine peak current amplitude (B); 10–90% rise time (C); percent fast desensitization (D) over 4 s from peak amplitude; and weighted time constant of deactivation (E). Representative currents from a subset of transfection conditions are presented in A. *, p < 0.05; **, p < 0.01; ***, p < 0.001 versus 1:1:0 and †, p < 0.05; ††, p < 0.01; †††, p < 0.001 versus 1:1:1.