FIGURE 8.

Effect of cycloheximide on expression of WT POR or A287P in MC3T3 cells. Stably transfected MC3T3 cells overexpressing either WT or A287P POR were treated with 3 μg per ml of cycloheximide, a protein synthesis inhibitor, for 12 h. Left, level of WT or A287P POR upon treatment with cycloheximide (+) was compared with cells treated with vehicle (−) by immunoblotting. Equal amounts of whole cell lysate (15 μg) were loaded in each lane, and β-actin was used as the loading control. Experiment was repeated twice and showed the same result; figure shown is representative. Right, densitometric analysis of immunoblot: % expression of WT POR and A287P POR in 2T3 stable cells treated with cycloheximide (solid bars). Expression level is normalized against untreated cells (empty bars). Values are mean ± S.D. (n = 2).