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. 2016 Aug 5;291(39):20487–20502. doi: 10.1074/jbc.M116.716019

TABLE 1.

Modifications to standard purification protocol for A287P

Standard protocol A287P modification
Growth temperature 28 °C 23 °C
IPTG concentration 500 μm 62.5 μm
Growth time 24 h 48 h
Detergent concentration 0.1% Triton X-100 0.5% Triton X-100