FIGURE 1.

Spectroscopic characterization of the holo-HemO_α and HemO_in proteins. A, CD spectra of holo-HemO_wt (black line), HemO_α (blue line), and HemO_in (red line). Spectra were recorded in 20 mm potassium phosphate (pH 7.8) at 25 °C with a final protein concentration of 20 μm. B, thermal denaturation curves θ at 222 nm for holo-HemO_wt (black circles), HemO_α (blue circles), and HemO_in (red circles). Buffer and protein conditions as in A. C, absorbance spectra of 10 μm holo-HemO_wt (black lines), HemO_α (blue lines), and HemO_in (red lines). Buffer conditions are as in A.