FIGURE 8.

HasR mRNA and protein levels in PAO1 and the ΔhemO complemented strains. A, mRNA isolated at 0, 2, 5, and 7 h. mRNA values represent the standard deviation from three independent experiments performed in triplicate and normalized to PAO1 at 0 h. The indicated p values were normalized to mRNA levels of PAO1 at the same time point, where *, p < 0.05, and **, p < 0.005. B, representative Western blot of HasR. Total protein (25 μg) was loaded in each well. RNA polymerase α was used as an equal loading control. Normalized density (n = 3) was performed on Western blots for three separate biological replicates. The indicated p values were normalized to PAO1 at the same time point where *, p < 0.05, and **, p < 0.005.