FIGURE 3.

Renal and neurological phenotype. A, urine production measured in metabolic cages overnight and expressed as microliters of urine per hour normalized to body weight. B, overnight water intake measured in grams of water normalized to body weight. C, urea in plasma expressed in mg per deciliter. D, electrolytes measured in urine and normalized to grams of creatinine. E, Western blot of Mut and Lcn2 in kidney at two different time points. F, brain weight normalized to body weight at three different time points. G, expression of Lcn2 in brain tissue on mRNA level normalized to β-actin and on protein level (β-actin as loading control). In A–D, F, and G, points and bars represent mean values (error bars depict S.E.; *, p < 0.05).