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. 2016 Aug 12;291(39):20588–20601. doi: 10.1074/jbc.M116.740191

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Regulation of GDH2 expression by Mxr1p and Mxr1p^N400. A, schematic representation of Mxr1p and Mxr1p^N400. The major domains within the 400 N-terminal amino acids are indicated. B, MXR1 and MXR^N400 mRNA in different P. pastoris strains cultured in YP and YNB + Glu media as analyzed by qPCR. C, effect of overexpression of Mxr1p and Mxr1p^N400 on the growth of Δmxr1 cultured in YP and YNB + Glu media. D, Western blotting analysis of Gdh2p^His levels in Δmxr1 overexpressing Mxr1p and Mxr1p^N400. The gene encoding Gdh2p^His was expressed from its own promoter in different strains as indicated. E, quantitation of data presented in D. F, analysis of GDH2 mRNA levels by qPCR in different P. pastoris strains cultured in YP and YNB + Glu media. Error bars indicate mean ± S.D. *, p < 0.05; **, p < 0.005; ***, p < 0.0005; ns, not significant. One-way analysis of variance followed by Tukey's multiple comparison test was carried out (n = 3).