TABLE 1.
P. pastoris strains used in this study
| Strain | Genotype | Ref. |
|---|---|---|
| GS115 | his4 | 5 |
| Δmxr1 | GS115, Ppmxr1Δ::Zeocinr | 13 |
| Δprm1 | GS115, Ppprm1Δ::Zeocinr | 10 |
| Δrop1 | GS115, Pprop1Δ::Zeocinr | 9 |
| Δgdh2 | GS115, Ppgdh2Δ::Zeocinr | This study |
| Δaat1 | GS115, Ppaat1Δ::Zeocinr | This study |
| Δaat2 | GS115, Ppaat2Δ::Zeocinr | This study |
| Δmdh1 | GS115, Ppmdh1Δ::Zeocinr | This study |
| Δmdh2 | GS115, Ppmdh2Δ::Zeocinr | This study |
| Δgdh2-GDH2His | Δgdh2, his4:: (PGDH2PpGDH2-His) | This study |
| GS115-GDH2His | GS115, his4:: (PGDH2PpGDH2-His) | This study |
| Δmxr1-GDH2His | Δmxr1, his4:: (PGDH2PpGDH2-His) | This study |
| MXR1Myc-OE | Δmxr1, Blasticidinr:: (PGAPDHPpMXR1-Myc) | 13 |
| MXR1Myc-OE-GDH2His | MXR1Myc-OE, his4:: (PGDH2PpGDH2-His) | This study |
| MXR1N400-OE | Δmxr1, Blasticidinr:: (PGAPDHPpMXR1N400-Myc) | 13 |
| MXR1N400-OE-GDH2His | MXR1N400-OE, his4:: (PGDH2PpGDH2-His) | This study |
| Δmxr1-GDH2-OE1 | Δmxr1, Zeocinr:: (PGAPDHPpGDH2-Myc) | This study |
| Δmxr1-GDH2-OE2 | Δmxr1, Zeocinr:: (PCUPPpGDH2-Myc) | This study |
| AAT1Myc | GS115, Zeocinr:: (PAAT1PpAAT1-Myc) | This study |
| AAT2Myc | GS115, Zeocinr:: (PAAT2PpAAT2-Myc) | This study |
| MDH1His | GS115, his4:: (PMDH1PpGDH2-His) | This study |
| MDH2His | GS115, his4:: (PMDH2PpMDH2-His) | This study |
| GLN1Myc | GS115, Zeocinr:: (PGLN1PpGLN1-Myc) | This study |