TABLE 2.
Plasmids used in this study
| Plasmid | Description | Source/Ref. |
|---|---|---|
| pIB3 | P. pastoris expression vector with HIS4 selection marker | Addgene |
| pIB3-GDH2His | pIB3 expressing GDH2His from − 545 bp of P. pastoris GDH2 promoter | This study |
| pGAPZA | P. pastoris expression vector with GAPDH promoter and ZeocinR selection marker | Invitrogen |
| pGAPBA | Modified pGAPZA vector with GAPDH promoter and BlasticidinR selection marker | 13 |
| pGAPDH-GDH2Myc | pGAPZA vector expressing Myc-tagged GDH2 from GAPDH promoter | This study |
| pCUP-GDH2Myc | pGAPZA vector expressing Myc-tagged GDH2 from CUP1 promoter | This study |
| pGAPBA-MXR1Myc | pGAPBA vector expressing Mxr1p from GAPDH promoter | 13 |
| pGAPBA-MXR1N400 | pGAPBA vector expressing Mxr1pN400 from GAPDH promoter | 13 |
| pGAPZA-AAT1Myc | pGAPZA vector expressing Myc-tagged AAT1 from − 1000 bp of AAT1 promoter | This study |
| pGAPZA-AAT2Myc | pGAPZA vector expressing Myc-tagged AAT2 from − 1000 bp of AAT2 promoter | This study |
| pIB3-MDH1His | pIB3 vector expressing His-tagged MDH1 from − 1000 bp of MDH1 promoter | This study |
| pIB3-MDH2His | pIB3 vector expressing His-tagged MDH2 from − 1000 bp of MDH2 promoter | This study |
| pGAPZA-GLN1Myc | pGAPZA vector expressing Myc-tagged GLN1 from − 1000 bp of GLN1 promoter | This study |