FIGURE 2.

Mapping of the domains in MICAL3 required for the interaction with MKLP1. A, schematic map of the MICAL3-MKLP1 complex based on interprotein cross-links (red lines). B, schematic overview of MICAL3 deletion mutants used in this study and a summary of identified interactions. Numbering is based on MICAL3 isoform 1 (NP_056056). Binding regions for the known partners of MICAL3 are indicated. C–E, streptavidin pulldown assays performed with the extracts of HEK293T cells co-expressing BirA, BioGFP, or the indicated MICAL3 or MKLP1 constructs. Antibodies against GFP, mCherry, or MKLP1 were used for Western blotting, as indicated.