RadD interacts with DNA.
A, Electrophoretic mobility shift of 5-fold dilution series of RadD incubated with poly-dT100 oligonucleotide and no nucleotide, ATP, ADP, or ATPγS (adenosine 5′-O-(thiotriphosphate); 5 mm). B, electrophoretic mobility shift of 5-fold dilution series of RadD K37R incubated with poly-dT100 oligonucleotide with or without ATP (5 mm). C, electrophoretic mobility shift of RadD incubated with poly-dT100 oligonucleotide with increasing concentrations of SSB tail peptide with or without ATP (5 mm). D, fluorescence polarization assay of increasing concentrations of RadD incubated with fluorescent poly-dT100 oligonucleotide. E, RadD ATP hydrolysis rates in the presence of various concentrations of M13mp18 circular single-stranded DNA (css) in the presence or absence of full-length SSB (10 μm).