FIGURE 7.

Knockdown of endogenous NPM1 is toxic to otherwise healthy neurons. A, HT22 cells were transfected with either a control (pLK0.1) or three commercially available shRNAs targeting NPM1 (sh1, sh2, and sh3) for 72 h. The cells were then lysed and subjected to Western blotting analysis with an antibody against NPM1. β-Tubulin serves as a loading control. B, viability of CGN cultures transfected with a control (pLK0.1), sh1, or sh2 along with EGFP in a 6.5:1 ratio and treated with HK/LK medium. Viability was quantified by EGFP autofluorescence. ***, p < 0.001 as compared with pLK0.1 in HK (n = 3).