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. 2016 Sep 23;6:34188. doi: 10.1038/srep34188

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Copyright © 2016, The Author(s)

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Constitutive ectopic expression of CIBZ suppresses ESC differentiation: (a) Western blotting analyses of stable pEF1α (control) and pEF1α-CIBZ (CIBZ OE) transfected ESCs. Control #1 and CIBZ OE #3 clones were chosen for subsequent studies; OE, overexpression; (b,c) Expression levels of the indicated proteins and mRNAs in WT and CIBZ OE EBs were determined using immunoblotting (b) and qPCR (c) respectively; Data from qPCR experiments (c) were normalized to the expression of GAPDH; *p < 0.05; (d) A schematic presentation of differentiation strategies for induction of cardiomyocyte differentiation in ESCs; (e) The incidence of spontaneously beating EBs was quantified at the indicated time points during differentiation; N = 20, *p < 0.05.