Table 1. Primers and PCR conditions.
| Primer Name | Sequence (5′–3′) | Tm (°C) | No. of cycle |
|---|---|---|---|
| psbA qPCR forward | TGCCATTATTCCTACTTCTGCA | 60 | 30 |
| psbA qPCR reverse | AGCACTAAAAAGGGAGCCG | 60 | 30 |
| psaA qPCR forward | GCAGGGCTACTAGGACTTGG | 60 | 30 |
| psaA qPCR reverse | GGCCTGTAAATGGACCTTTATG | 60 | 30 |
| petA qPCR forward | CAGCAGAATTATGAAAATCCACG | 60 | 30 |
| petA qPCR reverse | TATTAGTAGCAGGGTCTGGAGCA | 60 | 30 |
| ATFD2 qPCR forward | ACTTCATTCATCCGTCGTTCC | 60 | 30 |
| ATFD2 qPCR reverse | AAGAACCAGCACGGCAAG | 60 | 30 |
| LHCB1.1 qPCR forward | CCGTGTGACAATGAGGAAGA | 60 | 30 |
| LHCB1.1 qPCR reverse | CAAACTGCCTCTCCAAACTTG | 60 | 30 |
| ATBCA3 qPCR forward | CGAGTTCATAGAAAACTGGATCC | 56 | 35 |
| ATBCA3 qPCR reverse | AGGCAGGGGTAGTCTTGAAGT | 56 | 35 |
| APX1 qPCR forward | GGACGATGCCACAAGGATA | 58 | 35 |
| APX1 qPCR reverse | GTATTTCTCGACCAAAGGACG | 58 | 35 |
| GST qPCR forward | TCTATAAAACACCATACCTTCCTTCA | 58 | 35 |
| GST qPCR reverse | CGAAAAGCGTCAAATCACC | 58 | 35 |
| PP2AAC qPCR forward | GCGGTTGTGGAGAACATGATACG | * | * |
| PP2AAC qPCR reverse | GAACCAAACACAATTCGTTGCTG | * | * |
*The annealing temperature and PCR cycles of PP2AAC ‘housekeeping’ gene is dependent on the PCR condition of the genes being studied.