Figure 6. Flow cytometric analysis of mTOR S2448 phosphorylation in primary leukocytes.
Primary leukocytes were incubated in the absence (UT) or in the presence of IL-6 stimulation (10 ng/ml) for 15 min and analyzed by flow cytometry. (a) MFI of mTOR S2448 phosphorylation measured in primary B cells. (b) MFI of mTOR S2448 phosphorylation measured in primary PMNs. (c) MFI of mTOR S2448 phosphorylation measured in primary monocytes. Data are mean ± SEM of five independent experiments performed in LCLs obtained from five different SDS patients and compared to five different healthy donors. Student’s t-test has been reported.