Table 2. Lectin staining, subjectively described from +++ (very strong) to − (absent) in rIL-25-treated and rIL-25-treated and infected mice with Echinostoma caproni metacercariae.
Lectin | rIL-25 treated mice | Exposed rIL-25 treated mice | |||||||
---|---|---|---|---|---|---|---|---|---|
dpt (dpi) | Brush border | Mucus | Goblet cell vesicles |
Brush border | Mucus | Goblet cell vesicles |
|||
Staining | Predominance | Staining | Predominance | ||||||
WGA | 0 (0) | − | − | − | NA | − | − | − | NA |
2 (0) | +/− | + | +/− | + | +/− | + | +/− | + | |
5 (3) | +/− | ++ | ++/− | ++ | + | + | +/− | + | |
9 (7) | ++/− | +++ | ++/− | ++ | +/− | + | +/− | − | |
16 (14) | +/− | ++ | ++/− | ++ | +/− | +/− | +/− | − | |
DBA | 0 (0) | ++ | ++ | ++ | NA | ++ | ++ | ++ | NA |
2 (0) | ++ | ++ | ++/− | ++ | ++ | ++ | ++/− | + | |
5 (3) | ++ | ++ | ++/− | ++ | ++ | ++ | ++ | NA | |
9 (7) | +++ | +++ | +++ | NA | ++ | +++ | ++ | NA | |
16 (14) | ++ | ++ | ++/− | ++ | + | + | +/− | + | |
UEA-I | 0 (0) | − | ++ | + | NA | − | ++ | + | NA |
2 (0) | − | +++ | +++/− | +++ | − | +++ | +++/− | +++ | |
5 (3) | − | +++ | +++/− | +++ | − | ++/− | ++/− | ++ | |
9 (7) | − | +++ | +++/− | +++ | +/− | + | + | NA | |
16 (14) | − | +++ | +++/− | +++ | − | + | +/− | − | |
SNA | 0 (0) | − | + | +/− | − | − | + | +/− | − |
2 (0) | − | + | +/− | − | − | + | +/− | − | |
5 (3) | − | ++/− | ++/− | ++ | − | +/− | +/− | +/− | |
9 (7) | − | ++/− | ++/− | ++ | − | ++/− | ++/− | ++ | |
16 (14) | − | + | +/− | − | − | +/− | +/− | − |
The staining was independently evaluated in brush border, mucus and goblet cell vesicles. In case that both stained and non-stained vesicles were observed, the predominant ones are indicated in the right column. When all vesicles showed the same staining pattern this distinction is not applicable (NA). dpt: day after starting with intraperitoneal administration of IL-25; dppi: day after challenge with Echinostoma caproni metacercariae.