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. 2016 Sep 23;6:33924. doi: 10.1038/srep33924

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) The human IGH locus with details of BAC clones used as 3D FISH probes⁴⁴,⁴⁶. (B) Spatial distances separating distal Vh, proximal Vh, and Ch regions in precursor-B cells from fetal and pediatric BM. Grey dots represent individual alleles, red bars median values. Per group, >200 alleles from 2 donors were analyzed. Statistics: Mann–Whitney U test; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. (C) Representative images of stained IGH loci.