Figure 2. Apoptosis is reduced in olfactory mucosa after 8 weeks of HFruD.
Bar graphs represent relative quantification (mean ± SEM) of fluorescent labelling for control and HFruD animals. (a) Apoptosis labelling using an antibody against cleaved caspase 3. (a1–4) Representative cleaved caspase 3 labelling (green) images. Scale bars: 50 μm. (a5) Quantification of relative fluorescence of cleaved caspase 3 labelling after 4/8 weeks of diet (Control n = 4/7 animals, HFruD n = 6/8 animals). **p < 0.01 after Mann-Whitney’s test. (b) Cell proliferation labelling using an antibody against PCNA. (b1–4) Representative PCNA labelling (green) images. Scale bars: 50 μm. (b5) Quantification of relative fluorescence of PCNA labelling after 4/8 weeks of diet (control n = 5/7 animals, HFruD n = 6/7 animals).