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. 2016 Sep 23;6:33990. doi: 10.1038/srep33990

Figure 5. Zephycandidine A (1) induces apoptosis in leukemia cells.

Figure 5

(A) Morphological changes of HL-60 cells after treated with zephycandidine A (1) for 48 h. Phase-contrast microscopic view, magnification 400 × . (B) Apoptosis was determined by Annexin V-FITC and PI staining using flow cytometric analysis. Cells in the lower right quadrant indicate early apoptotic cells, and cells in the upper right quadrant indicate late apoptotic cells. (C) Western blot analysis for PARP, Bcl-2, Bax and caspase-3 levels, β-Actin used as a loading control. The relative density of each protein was detected by Image J, data are presented as the means of three experiments, bars, SD.