Figure 6.

(A) TIRFM image for ${\theta }_1$ = 63.6° (acquisition rate = 100 ms). (B–F) Data processing of the vaTIRFM images: $z_0$ image (B) and the corresponding distance histogram (C), $n_{\text{eff}}$ image (D), $\Delta z_0$ image (E), and the corresponding histogram (F). From image (B), the mean membrane/substrate distance is $\langle z_0\rangle$ = 120 nm and the standard deviation is ${\sigma }_{z_0}$ = 42 nm. From image (E), the mean $z_0$ error is $\langle \Delta z_0\rangle$ = 13 nm and the standard deviation is ${\sigma }_{\Delta z_0}$ = 5 nm. Scale bar = 20 μm. (B) The red arrow indicates the direction of migration. To see this figure in color, go online.