Figure 4.

Comparison of the performance of the local mean, median, and LSP background estimators in different molecular environments (experimental data). (a) Schematic representation of the molecule aperture and the background aperture surrounding the molecule. The aperture width is the width of the inner aperture. (b and c) Multiaperture signatures of representative molecules with varying local density. The corrected intensity is the background-subtracted donor intensity until the time of bleaching as a function of the molecule aperture size. The background is the total donor background intensity until the time of bleaching. (b) Single isolated molecule. (c) Molecule in regions where a neighbor is located within a distance of 5–10 pixels from the molecule of interest. In both cases, the aperture is centered on the highest-intensity pixel in the spot of the molecule under consideration. To see this figure in color, go online.